Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil.
Identifieur interne : 000066 ( Main/Exploration ); précédent : 000065; suivant : 000067Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil.
Auteurs : Ines Zrafi-Nouira [Tunisie] ; Sonda Guermazi ; Rakia Chouari ; Nimer M D. Safi ; Eric Pelletier ; Amina Bakhrouf ; Dalila Saidane-Mosbahi ; Abdelghani SghirSource :
- Biodegradation [ 1572-9729 ] ; 2009.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (génétique), Bactéries (croissance et développement), Bactéries (isolement et purification), Bactéries (métabolisme), Chromatographie en phase gazeuse (MeSH), Eau de mer (microbiologie), Microbiologie de l'eau (MeSH), Pétrole (métabolisme), Réaction de polymérisation en chaîne (MeSH), Spécificité d'espèce (MeSH).
- MESH :
- croissance et développement : Bactéries.
- génétique : ARN ribosomique 16S.
- isolement et purification : Bactéries.
- microbiologie : Eau de mer.
- métabolisme : Bactéries, Pétrole.
- Chromatographie en phase gazeuse, Microbiologie de l'eau, Réaction de polymérisation en chaîne, Spécificité d'espèce.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical , metabolism : Petroleum.
- growth & development : Bacteria.
- isolation & purification : Bacteria.
- metabolism : Bacteria.
- microbiology : Seawater.
- Chromatography, Gas, Polymerase Chain Reaction, Species Specificity, Water Microbiology.
Abstract
The indigenous microbiota of polluted coastal seawater in Tunisia was enriched by increasing the concentration of zarzatine crude oil. The resulting adapted microbiota was incubated with zarzatine crude oil as the only carbon and energy source. Crude oil biodegradation capacity and bacterial population dynamics of the microbiota were evaluated every week for 28 days (day 7, day 14, day 21, and day 28). Results show that the percentage of petroleum degradation was 23.9, 32.1, 65.3, and 77.8%, respectively. At day 28, non-aromatic and aromatic hydrocarbon degradation rates reached 92.6 and 68.7%, respectively. Bacterial composition of the adapted microflora was analysed by 16S rRNA gene cloning and sequencing, using total genomic DNA extracted from the adapted microflora at days 0, 7, 14, 21, and 28. Five clone libraries were constructed and a total of 430 sequences were generated and grouped into OTUs using the ARB software package. Phylogenetic analysis of the adapted microbiota shows the presence of four phylogenetic groups: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Diversity indices show a clear decrease in bacterial diversity of the adapted microflora according to the incubation time. The Proteobacteria are the most predominant (>80%) at day 7, day 14 and day 21 but not at day 28 for which the microbiota was reduced to only one OTU affiliated with the genus Kocuria of the Actinobacteria. This study shows that the degradation of zarzatine crude oil components depends on the activity of a specialized and dynamic seawater consortium composed of different phylogenetic taxa depending on the substrate complexity.
DOI: 10.1007/s10532-008-9235-x
PubMed: 19052881
Affiliations:
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Le document en format XML
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The resulting adapted microbiota was incubated with zarzatine crude oil as the only carbon and energy source. Crude oil biodegradation capacity and bacterial population dynamics of the microbiota were evaluated every week for 28 days (day 7, day 14, day 21, and day 28). Results show that the percentage of petroleum degradation was 23.9, 32.1, 65.3, and 77.8%, respectively. At day 28, non-aromatic and aromatic hydrocarbon degradation rates reached 92.6 and 68.7%, respectively. Bacterial composition of the adapted microflora was analysed by 16S rRNA gene cloning and sequencing, using total genomic DNA extracted from the adapted microflora at days 0, 7, 14, 21, and 28. Five clone libraries were constructed and a total of 430 sequences were generated and grouped into OTUs using the ARB software package. Phylogenetic analysis of the adapted microbiota shows the presence of four phylogenetic groups: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Diversity indices show a clear decrease in bacterial diversity of the adapted microflora according to the incubation time. The Proteobacteria are the most predominant (>80%) at day 7, day 14 and day 21 but not at day 28 for which the microbiota was reduced to only one OTU affiliated with the genus Kocuria of the Actinobacteria. This study shows that the degradation of zarzatine crude oil components depends on the activity of a specialized and dynamic seawater consortium composed of different phylogenetic taxa depending on the substrate complexity.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Bakhrouf, Amina" sort="Bakhrouf, Amina" uniqKey="Bakhrouf A" first="Amina" last="Bakhrouf">Amina Bakhrouf</name><name sortKey="Chouari, Rakia" sort="Chouari, Rakia" uniqKey="Chouari R" first="Rakia" last="Chouari">Rakia Chouari</name><name sortKey="Guermazi, Sonda" sort="Guermazi, Sonda" uniqKey="Guermazi S" first="Sonda" last="Guermazi">Sonda Guermazi</name><name sortKey="Pelletier, Eric" sort="Pelletier, Eric" uniqKey="Pelletier E" first="Eric" last="Pelletier">Eric Pelletier</name><name sortKey="Safi, Nimer M D" sort="Safi, Nimer M D" uniqKey="Safi N" first="Nimer M D" last="Safi">Nimer M D. Safi</name><name sortKey="Saidane Mosbahi, Dalila" sort="Saidane Mosbahi, Dalila" uniqKey="Saidane Mosbahi D" first="Dalila" last="Saidane-Mosbahi">Dalila Saidane-Mosbahi</name><name sortKey="Sghir, Abdelghani" sort="Sghir, Abdelghani" uniqKey="Sghir A" first="Abdelghani" last="Sghir">Abdelghani Sghir</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Zrafi Nouira, Ines" sort="Zrafi Nouira, Ines" uniqKey="Zrafi Nouira I" first="Ines" last="Zrafi-Nouira">Ines Zrafi-Nouira</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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